954 Differential regulation of comedogenesis and folliculitis by free fatty acids metabolized by cutibacterium acnes: Comprehensive analysis of human acne subjects and animal model

Free fatty acid (FFA) is one of the pathogenic factors in acne vulgaris. However, how FFAs interact with skin microbiome and host immunity still unclear. To clarify this, we comprehensively investigated lesions developed a novel mouse model. First, measured severity amounts (C12-C24) healthy- acne-forehead (50 females, aged 20-40 years). The total palmitic (PA) were weakly correlated number inflammatory (Rs=0.299, Rs=0.297, p<0.05). We also performed 16SrRNA DNA analysis (OTU97) metatranscriptome analysis. abundance Cutibacterium acnes by was not different between healthy subjects. In contrast, RNA expressions C. significantly elevated severe patients. Additionally, only metabolic pathways (Rs>0.4, p<0.05) positively FFAs. Then, roles FFA comedogenesis inflammation using When applied FAs various chain lengths (C12:0-C18:0) to ears, induced having more than 14 carbon atoms. gene keratinization markers, such as Krt16 Klk6 (p<0.01), depending on FAs. Interestingly, histological revealed that PA but no other folliculitis. Furthermore, strongly Cxcl2 (p<0.05). Our data suggest metabolized determine severity; atoms important eliciting